Vectors for improved Tet repressor-dependent gradual gene induction or silencing in Staphylococcus aureus.

@article{Helle2011VectorsFI,
  title={Vectors for improved Tet repressor-dependent gradual gene induction or silencing in Staphylococcus aureus.},
  author={Leonie Helle and Melanie Kull and Sonja Mayer and G. Marincola and M. Zelder and C. Goerke and C. Wolz and R. Bertram},
  journal={Microbiology},
  year={2011},
  volume={157 Pt 12},
  pages={
          3314-23
        }
}
  • Leonie Helle, Melanie Kull, +5 authors R. Bertram
  • Published 2011
  • Biology, Medicine
  • Microbiology
  • A set of vectors for improved tetracycline-dependent gene regulation in Staphylococcus aureus is presented. Plasmid pRAB11 was generated from pRMC2 by adding a second tet operator within the TetR-regulated promoter P(xyl/tet). Pronounced repression was observed in the absence of anhydrotetracycline (ATc) combined with high induction in the presence of the drug, as demonstrated for pRAB11 bearing staphylococcal nuclease nuc1, lacZ or gfp. Also, in plasmid pCG261, the pRAB11 tetR-P(xyl/tet… CONTINUE READING
    58 Citations
    A tetracycline‐inducible integrative expression system for Streptococcus pneumoniae
    • 1
    • PDF
    Adaption of an Episomal Antisense Silencing Approach for Investigation of the Phenotype Switch of Staphylococcus aureus Small-Colony Variants
    • Highly Influenced
    • PDF
    Construction of a Gene Knockdown System Based on Catalytically Inactive (“Dead”) Cas9 (dCas9) in Staphylococcus aureus
    • 17
    • PDF
    Revisiting the regulation of the capsular polysaccharide biosynthesis gene cluster in Staphylococcus aureus
    • 3
    Complementation Plasmids, Inducible Gene-Expression Systems, and Reporters for Staphylococci.
    • R. Bertram
    • Biology, Medicine
    • Methods in molecular biology
    • 2016
    Staphylococcus aureus competence genes: mapping of the SigH, ComK1 and ComK2 regulons by transcriptome sequencing
    • 24
    • Highly Influenced

    References

    SHOWING 1-10 OF 49 REFERENCES
    New Architectures for Tet-On and Tet-Off Regulation in Staphylococcus aureus
    • 22
    • PDF
    Improved tetracycline repressors for gene silencing in mycobacteria
    • 87
    • PDF
    The application of Tet repressor in prokaryotic gene regulation and expression
    • 104
    Tetracycline-Dependent Conditional Gene Knockout in Bacillus subtilis
    • 41
    • PDF
    Silencing Essential Protein Secretion in Mycobacterium smegmatis by Using Tetracycline Repressors
    • 98
    • PDF
    Gene regulation by tetracyclines. Constraints of resistance regulation in bacteria shape TetR for application in eukaryotes.
    • 133
    • Highly Influential
    • PDF
    Inducible Antisense RNA Expression in the Characterization of Gene Functions in Streptococcus mutans
    • 40
    • PDF
    An improved tetracycline-inducible expression vector for Staphylococcus aureus.
    • 130
    • Highly Influential