Vectors for Transformation of Higher Plants

@article{Klee1985VectorsFT,
  title={Vectors for Transformation of Higher Plants},
  author={Harry J. Klee and Martin F. Yanofsky and Eugene W. Nester},
  journal={Bio/Technology},
  year={1985},
  volume={3},
  pages={637-642}
}
We have constructed several vectors for the cloning of foreign DNA into plant genomes. These vectors are based on the transformation system of Agrobacterium tumefaciens and incorporate a number of useful features for plant cell transformation. The prototype vector, pEND4K, contains the left and right T-DNA border sequences and a chimeric gene which confers kanamycin resistance on transformed plant cells. The vector also includes the following features that facilitate the bacterial manipulations… CONTINUE READING

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