Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli.

@article{Amann1983VectorsBA,
  title={Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli.},
  author={Egon P. Amann and J{\"u}rgen Brosius and Mark Ptashne},
  journal={Gene},
  year={1983},
  volume={25 2-3},
  pages={167-78}
}
A strong promoter has been cloned on a series of plasmid vectors that facilitate the expression of cloned genes. This promoter, named tac [first described by DeBoer et al. (in Rodriguez, R.L. and Chamberlin, M.J. (Eds.),Promoters, Structure and Function. Praeger, New York, 1982, pp. pp. 462-481)] contains the -10 region of the lacUV5 promoter and the -35 region of the trp promoter. Our vectors contain various cloning sites followed by transcription termination signals. In addition, we describe… CONTINUE READING
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