Vector-free cloning of a bacterial endo-1,4-β-glucanase in Lactobacillus plantarum and its effect on the acidifying activity in silage: Use of recombinant cellulolytic Lactobacillus plantarum as silage inoculant

@article{Rossi2001VectorfreeCO,
  title={Vector-free cloning of a bacterial endo-1,4-β-glucanase in Lactobacillus plantarum and its effect on the acidifying activity in silage: Use of recombinant cellulolytic Lactobacillus plantarum as silage inoculant},
  author={Franca Rossi and Andrea Rudella and Marta Marzotto and Franco Dellaglio},
  journal={Antonie van Leeuwenhoek},
  year={2001},
  volume={80},
  pages={139-147}
}
In this research, the advantage of use of cellulolytic recombinant Lactobacillus plantarum as microbial inoculants for alfalfa silage fermentation was evaluated. To such purpose, two L. plantarum strains, one (L. plantarum Lp80) currently commercialised and the other (L. plantarum B41) suitable as silage microbial additive, were genetically modified by integration of celA gene, encoding an alkaline endo-1,4-β-glucanase from Bacillus sp., in the chromosome, by means of a vector-free cloning… CONTINUE READING
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