• DOI:10.1186/1743-7075-7-46
• Corpus ID: 819167

Vaticanol C, a resveratrol tetramer, activates PPARα and PPARβ/δ in vitro and in vivo

@article{Tsukamoto2010VaticanolCA,
  title={Vaticanol C, a resveratrol tetramer, activates PPAR$\alpha$ and PPAR$\beta$/$\delta$ in vitro and in vivo},
  author={Tomoko Tsukamoto and Rieko Nakata and Emi Tamura and Yukiko Kosuge and Aya Kariya and Michiko Katsukawa and Satoshi Mishima and Tetsuro Ito and Munekazu Iinuma and Yukihiro Akao and Yoshinori Nozawa and Yuji Arai and Shobu Namura and Hiroyasu Inoue},
  journal={Nutrition \& Metabolism},
  year={2010},
  volume={7},
  pages={46 - 46}
}

• T. Tsukamoto, R. Nakata, +11 authors H. Inoue
• Published 27 May 2010
• Biology
• Nutrition & Metabolism

BackgroundAppropriate long-term drinking of red wine is associated with a reduced risk of cardiovascular disease. Resveratrol, a well-known SIRT1 activator is considered to be one of the beneficial components contained in red wine, and also developed as a drug candidate. We previously demonstrated that resveratrol protects brain against ischemic stroke in mice through a PPARα-dependent mechanism. Here we report the different effects of the oligomers of resveratrol.MethodsWe evaluated the activation of PPARs by ε-viniferin, a resveratrol dimer, and vaticanol C, a resveratrol tetramer, in cell-based reporter assays using bovine arterial endothelial cells, as well as the activation of SIRT1. Moreover, we tested the metabolic action by administering vaticanol C with the high fat diet to wild-type and PPARα-knockout male mice for eight weeks.ResultsWe show that vaticanol C activates PPARα and PPARβ/δ in cell-based reporter assays, but does not activate SIRT1. ε-Viniferin shows a similar radical scavenging activity as resveratrol, but neither effects on PPARs and SIRT-1. Eight-week intake of vaticanol C with a high fat diet upregulates hepatic expression of PPARα-responsive genes such as cyp4a10, cyp4a14 and FABP1, and skeletal muscle expression of PPARβ/δ-responsive genes, such as UCP3 and PDK4 (pyruvate dehydrogenase kinase, isoform 4), in wild-type, but not PPARα-knockout mice.ConclusionVaticanol C, a resveratrol tetramer, activated PPARα and PPARβ/δ in vitro and in vivo. These findings indicate that activation of PPARα and PPARβ/δ by vaticanol C may be a novel mechanism, affording beneficial effects against lifestyle-related diseases.