Vasopressin V2 receptor expression along rat, mouse, and human renal epithelia with focus on TAL.

  title={Vasopressin V2 receptor expression along rat, mouse, and human renal epithelia with focus on TAL.},
  author={Kerim Mutig and Alexander Paliege and Thomas Kahl and Thomas Jöns and Werner M{\"u}ller-Esterl and Sebastian Bachmann},
  journal={American journal of physiology. Renal physiology},
  volume={293 4},
In renal epithelia, vasopressin influences salt and water transport, chiefly via vasopressin V(2) receptors (V(2)Rs) linked to adenylyl cyclase. A combination of vasopressin-induced effects along several distinct portions of the nephron and collecting duct system may help balance the net effects of antidiuresis in cortex and medulla. Previous studies of the intrarenal distribution of V(2)Rs have been inconclusive with respect to segment- and cell-type-related V(2)R expression. Our study… 

Demonstration of the functional impact of vasopressin signaling in the thick ascending limb by a targeted transgenic rat approach.

The antidiuretic hormone vasopressin (AVP) regulates renal salt and water reabsorption along the distal nephron and collecting duct system. These effects are mediated by vasopressin 2 receptors (V2R)

The physiological and pathophysiological functions of renal and extrarenal vasopressin V2 receptors.

Current evidence on the physiological and pathophysiological functions of renal and extrarenal V2Rs is reviewed, finding that these functions are important, not only in rare diseases with loss or gain of function of V2R but also in relation to the recent use of nonpeptide V 2R antagonists to treat hyponatremia.

Vasopressin Increases Urinary Acidification via V1a Receptors in Collecting Duct Intercalated Cells.

Results show that activation of V1aR contributes to urinary acidification via H+ secretion by A-ICs, which may have clinical implications for pharmacologic targeting of V 1aR.

Group VIA phospholipase A2 is a target for vasopressin signaling in the thick ascending limb.

Microarray-based gene expression analysis and administration of the V2 receptor-selective agonist desmopressin to AVP-deficient diabetes insipidus rats increased outer medullary phosphorylated NKCC2 (pNKCC2) levels more than twofold in association with a marked reduction in iPLA(2)β abundance, suggesting that downregulation ofiPLA( 2)β may be a relevant step in A VP-mediated urine concentration.

Cyclophosphamide-induced vasopressin-independent activation of aquaporin-2 in the rat kidney.

It is demonstrated that, in the rat kidney, cyclophosphamide may activate V2R and induce upregulation of AQP2 in the absence of vasopressin stimulation, suggesting the possibility of drug-induced nephrogenic syndrome of inappropriate antidiuresis (NSIAD).

Kidney collecting duct cells make vasopressin in response to NaCl-induced hypertonicity

Evidence of biologically active production of kidney-derived vasopressin in kidney tubular epithelial cells is provided and it is found that murine collecting duct cells make biologically active vasoppressin which increases in response to NaCl mediated hypertonicity.

Vasopressin V2 receptors, ENaC, and sodium reabsorption: a risk factor for hypertension?

This review summarizes the effect of vasopressin V2 receptor stimulation on ENaC activity and sodium excretion in vivo and reports the experimental and clinical data demonstrating the role of renal ENoC in water conservation at the expense of a reduced ability to excrete sodium.

Short-term stimulation of the thiazide-sensitive Na+-Cl- cotransporter by vasopressin involves phosphorylation and membrane translocation.

The results suggest that the vasopressin-V(2) receptor-NCC signaling cascade is a novel effector system to adjust transepithelial NaCl reabsorption in DCT.

Axial heterogeneity of vasopressin-receptor subtypes along the human and mouse collecting duct.

Heterogenous axial expression of both V1a and V2 vasopressin receptors along the human and mouse collecting ducts are demonstrated, suggesting distinct regulation of renal transport function by AVP through V1anion exchanger-1-labeled alpha-intercalated cells in the cortex vs. the medulla.

Differential distribution of the vasopressin V receptor along the rat nephron during renal ontogeny and maturation.

expression of V(2)R is first detectable in the late embryonic stage of rat ontogeny starting from day E18 and gradually increasing with kidney maturation, and in the adult kidney, V( 2)r is differentially distributed in the various nephron segments.

Long-term effects of vasopressin on the subcellular localization of ENaC in the renal collecting system.

The findings suggest that the previously reported increase in sodium transport induced by sustained stimulation of vasopressin V2 receptor is probably mediated by other mechanism than an increase in the apical density of ENaC.

Regulation of Acid-Base Transporters by Vasopressin in the Kidney Collecting Duct of Brattleboro Rat

Chronic administration of dDAVP to Brattleboro rats is associated with the upregulation of PDS and downregulation of H+-ATPase and AE1 in the collecting duct, along with increased ammoniagenesis.

Chronic Exposure to Vasopressin Upregulates ENaC and Sodium Transport in the Rat Renal Collecting Duct and Lung

It is shown that vasopressin increases sodium transport in the renal collecting duct and probably in the lung, through a differential transcriptional regulation of ENaC subunits, which could lead to less efficient sodium excretion and thus participate in some forms of salt-sensitive hypertension.

Regulation of the Abundance of Renal Sodium Transporters and Channels by Vasopressin

Investigating the regulation of abundance of the major sodium transporters and channels expressed along the renal tubule in response to vasopressin found a significant increase in protein abundance for NKCC2 and the beta- and gamma-subunits of ENaC with either water restriction or dDAVP infusion.

Physiological effects of vasopressin and atrial natriuretic peptide in the collecting duct.

Vasopressin increases Na-K-2Cl cotransporter expression in thick ascending limb of Henle's loop.

It is concluded that vasopressin strongly upregulates the expression of the Na-K-2Cl cotransporter of the TAL and that it is likely to play an important role in the long-term regulation of the countercurrent multiplication system.

Immunohistochemical localization of V2 vasopressin receptor along the nephron and functional role of luminal V2 receptor in terminal inner medullary collecting ducts.

Data show that V2 receptors are localized not only in the basolateral membrane but also in the luminal membrane of the distal nephron, and luminal AVP acts as a negative feedback system upon the Basolateral action of AVP in tIMCD.

Cloning and characterization of a vasopressin V2 receptor and possible link to nephrogenic diabetes insipidus

The cloning of the rat kidney V2 AVP receptor complementary DNA that encodes a 370-amino-acid protein with a transmembrane topography characteristic of G protein-coupled receptors, and with similarity to the Via (hepatic) AVP receptors2 in its seven membrane-spanning domains is reported.