Vasoactive intestinal peptide: Expression of the prohormone in bacterial cells

  title={Vasoactive intestinal peptide: Expression of the prohormone in bacterial cells},
  author={J. Delamarter and G. Buell and E. Kawashima and J. Polak and S. Bloom},
  • J. Delamarter, G. Buell, +2 authors S. Bloom
  • Published 1985
  • Biology, Medicine
  • Peptides
  • A complementary DNA (cDNA) to the messenger RNA for the preprohormone of human vasoactive intestinal peptide (VIP) has been isolated and characterized. This cDNA extends from 65 bases 5' of the AUG translation start codon through the entire 3' untranslated region. Using this cDNA we have constructed expression plasmids which allow the synthesis of 120 out of the 150 amino acids of the prohormone in E. coli. This portion of the prohormone gene was either fused to a segment of a bacteriophage… CONTINUE READING
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