Vasoactive intestinal peptide: Expression of the prohormone in bacterial cells

  title={Vasoactive intestinal peptide: Expression of the prohormone in bacterial cells},
  author={John F. DeLamarter and Gary N. Buell and E. Kawashima and Julia M. Polak and Stephen Robert Bloom},
16 Citations

Early stages of phylogenesis of peptide regulation

The author’s concepts according to which elements of the system of peptide regulation are exclusively peptides emerging as a results of cleavage of ribosomally synthesized proteins-precursors are not the part of the above-mentioned system.

Characterization of vasoactive intestinal peptide receptors on human megakaryocytes and platelets.

Results suggest that VIP may have direct effects on megakaryocytopoiesis and support the earlier observations of VIP modulation of platelet aggregation.

Immunohistochemical localization of opioid peptides in the brain of the leech Theromyzon tessulatum

No correspondence could be established between the neurons containing met- or leu-enkephalin-like substances and the different types of neurosecretory cells classically described in Hirudinea.

Molecular cloning of five messenger RNAs differentially expressed in preneoplastic or neoplastic JB6 mouse epidermal cells: one is homologous to human tissue inhibitor of metalloproteinases-3.

This is the first report, to the authors' knowledge, of the simultaneous display of mRNAs of four phenotypically distinct cell variants and of the isolation of five clones which may be associated with specific stages of tumor promotion and/or progression and apoptosis.

Neuronal control of skin function: the skin as a neuroimmunoendocrine organ.

Together, a close multidirectional interaction between neuromediators, high-affinity receptors, and regulatory proteases is critically involved to maintain tissue integrity and regulate inflammatory responses in the skin.

Presynaptic opioid receptors modulating acetylcholine release in the hippocampus of the rabbit

It is concluded that the release of acetylcholine in the hippocampus of the rabbit is inhibited at the level of the axon terminals via κ-receptors; in addition, μ-receptionors may be present.



Cloning and sequence analysis of cDNA for the precursor of human growth hormone-releasing factor, somatocrinin.

In vitro translation of tumor poly(A)+ RNA followed by immunoprecipitation with GRF-specific antiserum and gel electrophoresis showed the molecular weight of preprosomatocrinin to be approximately 13,000, which is in good agreement with the Molecular weight deduced from the sequences of the cDNA clones.

Partial nucleotide sequence of human calcitonin precursor mRNA identifies flanking cryptic peptides

It is demonstrated by nucleotide sequence analysis of these cloned cDNA sequences that human calcitonin resides towards the carboxy terminal of a precursor polyprotein, and is flanked at the amino and car boxy termini by cryptic peptide sequences of unknown biological significance.

Biosynthesis of caerulein in the skin of Xenopus laevis: partial sequences of precursors as deduced from cDNA clones.

The decapeptide caerulein represents one of the main constituents of the skin secretion of Xenopus laevis and must be cleaved by an unusual processing mechanism to serve as the signal for amidation.

Nucleotide sequence of cloned cDNA encoding bovine arginine vasopressin–neurophysin II precursor

The sequence of a cDNA encoding the nonapeptide arginine vasopressin (A VP) and its carrier protein, neurophysin II (NpII) from bovine hypothalamus, proves that the 166-amino acid precursor molecule

Human preprovasoactive intestinal polypeptide contains a novel PHI-27-like peptide, PHM-27

The entire amino acid sequence of the precursor, deduced from the nucleotide sequence, indicates that the precursor protein contains not only VIP but also a novel peptide of 27 amino acids.

Cloning and sequence analysis of cDNA for ovine corticotropin-releasing factor precursor

Comparison of the amino acid sequence of prepro-CRF with those of the ACTH–β-LPH precursor and the arginine vasopressin–neurophysin II precursor suggests that these precursor proteins may be evolutionarily related.

In vitro synthesis of protein in microbial systems.

  • G. Zubay
  • Biology, Chemistry
    Annual review of genetics
  • 1973
This review is devoted to studies involving synthesis of complete proteins in cell-free systems, and a number of laboratories have studied the DNA-dependent RNA­ directed synthesis of both viral and bacterial proteins in E. coli extracts.

Cell-free transcription and translation of total spinach chloroplast DNA.

Evidence for the synthesis of more than 20 discrete size classes of [35S]-methionine-labelled polypeptides has been obtained by dodecylsulphate/polyacrylamide gel electrophoresis of the products formed in a reaction programmed with spinach chloroplast DNA.