Vasoactive Intestinal Peptide Gene: Putative Mechanism of Information Storage at the RNA Level

@article{Gozes1987VasoactiveIP,
  title={Vasoactive Intestinal Peptide Gene: Putative Mechanism of Information Storage at the RNA Level},
  author={Illana Gozes and Eliezer Giladi and Yael Shani},
  journal={Journal of Neurochemistry},
  year={1987},
  volume={48}
}
Abstract: The human gene coding for vasoactive intestinal peptide was recently isolated and shown to contain seven exons. We now demonstrate that an intron‐containing precursor RNA can be the major vasoactive intestinal peptide‐related RNA in the cell, which is in contrast to most known genes. By RNA blot analysis using a variety of genomic and cDNA‐related probes we show that in a human tumor producing vasoactive intestinal peptide, most of the RNA encoding the peptide is of the precursor type… 

Expression of messenger ribonucleic acids encoding neuropeptide-Y, substance-P, and vasoactive intestinal polypeptide in human pituitary.

The low levels of NPY, SP, and VIP and their mRNAs in the human pituitary are consistent with peptides having an autocrine/paracrine, rather than endocrine, mode of action.

Expression of mRNA for vasoactive intestinal peptide in normal human colon and during inflammation

The observations suggest that decreased vasoactive intestinal peptide gene expression or abnormal post-transcriptional processing are not primary defects in this disorder of chronic inflammation, and support the alternative hypothesis that axonal degeneration in ulcerative colitis could result in increased expression of neuronal vaso active intestinal peptides mRNA.

VIP: Molecular biology and neurobiological function

VIP is a major factor in brain activity, neuroendocrine functions, cardiac activity, respiration, digestion, and sexual potency, and exerts its function via receptor-mediated systems, activating signal transduction pathways, including cAMP.

Regulation of VIP gene expression in general

Results indicated that PMA treatment may induce both VIP mRNA synthesis as well as VIP mRNA stabilization, and suggested a 4–5 h half-life for the VIP mRNA in the absence of PMA, suggesting lung cancer tumor proliferation may be regulated, in part, at the level of VIP gene expression.

Detection of vasoactive intestinal polypeptide messenger RNA in ganglioneuroblastoma by in situ hybridization

Direct evidence is presented at the single cell level for the production of VIP by ganglionic cells in ganglioneuroblastoma as consistent with the localization of VIP-like immunoreactivity in the same tumor tissues.

Estrogen regulation of vasoactive intestinal peptide mRNA in rat hypothalamus

The results suggest a sexual dimorphism with regard to the steroid regulation of hypothalamic VIP gene expression in the rat.

Estrogen regulation of vasoactive intestinal peptide mRNA in rat hypothalamus

The results suggest a sexual dimorphism with regard to the steroid regulation of hypothalamic VIP gene expression in the rat.

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The pre-proVIP gene is isolated and the structure is determined, which appears to divide the gene into functional domains and one of the introns occurs within the 3'-untranslated region of the gene.

Detection of mRNAs containing regulatory peptide coding sequences using synthetic oligodeoxynucleotides

This work identified and partially purified a ∼ 1600‐base long mRNA containing VIP related sequences which can be translated in vitro into VIP‐immunoreactive polypeptides and differs in size from a known VIP‐mRNA identified in human neuro‐blastoma cells, suggesting the possibility of different VIP‐ mRNAs in different cell types.

Coding sequences for vasoactive intestinal peptide and PHM-27 peptide are located on two adjacent exons in the human genome.

The occurrence of VIP and PHM-27 coding sequences on two separate exons of the human genome and the homology of their 3' splice site may allow alternative RNA processing as discussed below.

Human preprovasoactive intestinal polypeptide contains a novel PHI-27-like peptide, PHM-27

The entire amino acid sequence of the precursor, deduced from the nucleotide sequence, indicates that the precursor protein contains not only VIP but also a novel peptide of 27 amino acids.

Molecular cloning of a cDNA encoding human antihaemophilic factor

A complete copy of the mRNA sequences encoding human coagulation factor VIII:C has been cloned and expressed and has an obvious domain structure, contains sequence repeats and is structurally related to factor V and ceruloplasmin.