Vascular gene discovery using laser capture microdissection of human blood vessels and quantitative PCR.

@article{Stagliano2001VascularGD,
  title={Vascular gene discovery using laser capture microdissection of human blood vessels and quantitative PCR.},
  author={Nancy E. Stagliano and Amalia Carpino and Jeffrey S. Ross and Michael J. Donovan},
  journal={Annals of the New York Academy of Sciences},
  year={2001},
  volume={947},
  pages={344-9}
}
Recent approaches to candidate gene identification and cellular localization have included RNA derived from complex whole tissue profiling on cDNA microarrays followed by in situ hybridization with riboprobes. In this study, the Arcturus PixCell II laser capture microdissection (LCM) system, an argon-based laser-assisted method for the isolation of specific cell types from heterogeneous tissue samples, was used to microdissect the tunica media from normal human arteries and veins (n = 5 in each… CONTINUE READING