Varying label density allows artifact-free analysis of membrane-protein nanoclusters

Abstract

We present a method to robustly discriminate clustered from randomly distributed molecules detected with techniques based on single-molecule localization microscopy, such as PALM and STORM. The approach is based on deliberate variation of labeling density, such as titration of fluorescent antibody, combined with quantitative cluster analysis, and it thereby… (More)
DOI: 10.1038/nmeth.3897

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