Various applications of TALEN- and CRISPR/Cas9-mediated homologous recombination to modify the Drosophila genome

Abstract

Modifying the genomes of many organisms is becoming as easy as manipulating DNA in test tubes, which is made possible by two recently developed techniques based on either the customizable DNA binding protein, TALEN, or the CRISPR/Cas9 system. Here, we describe a series of efficient applications derived from these two technologies, in combination with… (More)
DOI: 10.1242/bio.20147682

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