Variations in the distribution of sugar residues in the zona pellucida as possible species-specific determinants of mammalian oocytes.

@article{Skutelsky1994VariationsIT,
  title={Variations in the distribution of sugar residues in the zona pellucida as possible species-specific determinants of mammalian oocytes.},
  author={Ehud Skutelsky and E Ranen and Ruth Shalgi},
  journal={Journal of reproduction and fertility},
  year={1994},
  volume={100 1},
  pages={35-41}
}
The lectin-binding patterns of mammalian zonae pellucidae were investigated to determine whether differences reflected their characteristic carbohydrate distribution patterns. Ovaries isolated from rodents (mouse, rat and hamster), rabbits, cats, dogs and pigs were fixed with glutaraldehyde and embedded in paraffin wax. Sections 5 microns, were deparaffinized, rehydrated and labelled with ten different biotinylated lectins as probes and avidin-biotin-peroxidase complex as visualant. The zonae… CONTINUE READING

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