Variation within the glycoprotein B gene of human cytomegalovirus is due to homologous recombination.

@article{Haberland1999VariationWT,
  title={Variation within the glycoprotein B gene of human cytomegalovirus is due to homologous recombination.},
  author={Michael Haberland and Ursula Meyer-Koenig and Frank T. Hufert},
  journal={The Journal of general virology},
  year={1999},
  volume={80 ( Pt 6)},
  pages={1495-500}
}
Human cytomegalovirus (HCMV) strains can be classified into different glycoprotein B (gB) genotypes. In a previous study, frequent intragenic variation of the gB gene was shown. The aim of this study was to analyse whether gB variation was due to homologous recombination. The gB gene of DNA extracts derived from the peripheral blood leukocytes of 14 immunosuppressed patients was amplified by PCR and cloned. Three variable sites of gB were analysed by restriction fragment analysis and DNA… CONTINUE READING