Variation of single-strand break yield with scavenger concentration for plasmid DNA irradiated in aqueous solution.

@article{Milligan1993VariationOS,
  title={Variation of single-strand break yield with scavenger concentration for plasmid DNA irradiated in aqueous solution.},
  author={Jamie R. Milligan and Joe A Aguilera and John F. Ward},
  journal={Radiation research},
  year={1993},
  volume={133 2},
  pages={151-7}
}
We have measured the yield of single-strand breaks (SSBs), induced by 137Cs gamma radiation as assayed by agarose gel electrophoresis, for three plasmids and SV40 DNA irradiated in aerobic aqueous solution. DNA SSBs are caused mainly by the hydroxyl radical under these conditions. To characterize the reactivity of DNA with the hydroxyl radical, we investigated the variation of the G value for SSBs [G(SSB)] with the concentration of added hydroxyl radical scavengers. We find that simple… CONTINUE READING

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