Variation of rDNA Internal Transcribed Spacer Sequences in Rhizoctonia cerealis

  title={Variation of rDNA Internal Transcribed Spacer Sequences in Rhizoctonia cerealis},
  author={Lei Ji and Chunju Liu and Li Zhang and Aixin Liu and Jinfeng Yu},
  journal={Current Microbiology},
Fifty-four single protoplast isolates (SPIs) were regenerated from three Rhizoctonia cerealis strains. A total of 169 rDNA-ITS regions were cloned and sequenced from these 54 SPIs. Variations in the ITS1 and ITS2 regions that flank the 5.8S gene were found within clones from the same strain, as well as within clones from the same SPI. These include variations in GC content and ITS length, and single-nucleotide polymorphisms (SNPs). The different strains and SPIs GC contents range from 40.25 to… CONTINUE READING
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