Variation in the safety of induced pluripotent stem cell lines

  title={Variation in the safety of induced pluripotent stem cell lines},
  author={Kyoko Miura and Yohei Okada and Takashi Aoi and Aki Okada and Kazutoshi Takahashi and Keisuke Okita and Masato Nakagawa and Michiyo Koyanagi and Koji Tanabe and Mari Ohnuki and Daisuke Ogawa and Eiji Ikeda and Hideyuki Okano and Shinya Yamanaka},
  journal={Nature Biotechnology},
We evaluated the teratoma-forming propensity of secondary neurospheres (SNS) generated from 36 mouse induced pluripotent stem (iPS) cell lines derived in 11 different ways. Teratoma-formation of SNS from embryonic fibroblast–derived iPS cells was similar to that of SNS from embryonic stem (ES) cells. In contrast, SNS from iPS cells derived from different adult tissues varied substantially in their teratoma-forming propensity, which correlated with the persistence of undifferentiated cells. 

Derivation of Neural Stem Cells from Mouse Induced Pluripotent Stem Cells.

This review focuses on embryoid body (EB)-mediated stem cell culture techniques used to derive NSCs from mouse induced pluripotent stem cells (iPSCs).

Purified Mesenchymal Stem Cells Are an Efficient Source for iPS Cell Induction

The choice of cell type most suitable for inducing high-quality iPS cells remains unclear and the poor efficiency of cell reprogramming and the uneven quality ofiPS cells are still important problems.

Induced Pluripotent Stem Cells and Their Potential for Basic and Clinical Sciences

The recent progresses iniPS cell reprogramming and iPS cell based therapy are summarized, and patient specific iPS cells as a disease model are described at length in the light of the literature.

Prospects for Induced Phiripotent Stem Cell-Derived Hepatocytes in Cell Therapy.

Recent advances and challenges in hepatocyte differentiation from iPS cells and their utility in cell therapy are discussed.

Induction of pluripotency by defined factors

These established induced pluripotent stem (iPS) cells have similar features to embryonic stem (ES) cells, including pluripotency and immortality, and provides unprecedented opportunities for regenerative medicine and drug discovery.

Induced pluripotent stem cells: An update

The breakthrough research offering induction of pluripotent status in somatic cells by direct reprogramming depends upon the genes used for induction.

The tumorigenicity of human embryonic and induced pluripotent stem cells

A rapidly accumulating body of evidence suggests that there are important genetic and epigenetic differences between these two cell types, which seem to influence their tumorigenicity.

Generation of induced pluripotent stem cells from somatic cells.

Progress toward the clinical application of patient-specific pluripotent stem cells.

The current state of iPS cell technology is described, including approaches by which they are generated and what is known about their biology, and the potential applications of these cells for disease modeling, drug discovery, and, eventually, cell replacement therapy are discussed.

Generation of Induced Pluripotent Stem Cells from Somatic Cells

This chapter looks back at the story of generation of induced pluripotent stem cells and discusses recent understanding of iPS cells with the latest data.



Generation of induced pluripotent stem cells from human blood.

The ability to reprogram cells from human blood will allow the generation of patient-specific stem cells for diseases in which the disease-causing somatic mutations are restricted to cells of the hematopoietic lineage.

Generation of Pluripotent Stem Cells from Adult Mouse Liver and Stomach Cells

Genetic lineage tracings suggest that iPS cells are generated by direct reprogramming of lineage-committed somatic cells and that retroviral integration into specific sites is not required.

Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells

It is demonstrated that reprogrammed pluripotent cells can be isolated from genetically unmodified somatic donor cells solely based upon morphological criteria.

Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells

Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors (OCT4, SOX2, NANOG,

Reprogramming of human somatic cells to pluripotency with defined factors

The data demonstrate that defined factors can reprogramme human cells to pluripotency, and establish a method whereby patient-specific cells might be established in culture.

Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes

It is shown that reprograming of juvenile human primary keratinocytes by retroviral transduction with OCT4, SOX2, KLF4 and c-MYC is at least 100-fold more efficient and twofold faster compared with reprogramming of human fibroblasts.

Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts

A modified protocol for the generation of iPS cells that does not require the Myc retrovirus is described and, with this protocol, significantly fewer non-iPS background cells are obtained, and theiPS cells generated were consistently of high quality.