Variation in the activities of enzymes associated with cell wall metabolism during a growth cycle of Candida albicans

  title={Variation in the activities of enzymes associated with cell wall metabolism during a growth cycle of Candida albicans},
  author={K. Barrett-Bee and J. Lees and W. Henderson},
  journal={Fems Microbiology Letters},
Exo-l , 3P-glucanase Activity in Candida albicans : Effect of the Yeast-to-mycelium Transition
Yeast cells of Candida albicans 1001 produced glucan-hydrolysing activity, most of which was due to an exo-l,3-P-glucanase. The enzyme was periplasmically located; it could be found in culture mediumExpand
Stability and zymogenic nature of chitin synthase fromCandida albicans
Gel chromatography of enzyme preparations suggested that zymogen was preferentially retarded on the columm matrices in comparison with active enzyme. Expand
Phenotypic and genotypic variation in methylases involved in type II restriction-modification systems in Helicobacter pylori.
All of the methylases studied were present in all major human population groupings, suggesting that their horizontal acquisition pre-dated the separation of these populations and points toward substantial strain-specific selection involving these loci. Expand
Characterization of (1,3)-beta-glucan synthase in Candida albicans: microsomal assay from the yeast or mycelial morphological forms and a permeabilized whole-cell assay.
The addition of detergent during mechanical breakage of yeast cells dramatically improved glucan synthase stability and activity, and Brij-35 significantly stabilized the enzyme, yielding a half-life of 5.6 d at 4 degrees C, compared with 0.9 d without detergent. Expand
Compounds active against cell walls of medically important fungi
  • R. Hector
  • Medicine, Biology
  • Clinical Microbiology Reviews
  • 1993
Compounds specifically interfering with chitin or beta-glucan synthesis have proven effective in studies of preclinical models of mycoses, though they appear to have a restricted spectrum of coverage. Expand
Chitin Synthase as a Chemotherapeutic Target
The fungal cell wall is a major and essential fungal structure, entirely lacking in animal cells, and thus an attractive chemotherapeutic target. Its biosynthesis is central to cell division andExpand
Screening for Antifungal Activity
The purpose of antifungal screening is not to define the therapeutic properties of an individual compound, but rather to indicate which of a large number of compounds or samples are worthy of furtherExpand
What are the roles of chitinases in the growing fungus
Inhibition has only been observed of spore germination, of cell separation in budding yeasts and of the action of a yeast toxin, and not of apical extension or branching, but the finding that chitinase activities in situ have different properties to those in cell homogenates, and appear to be protected from environmental stresses is explained. Expand
A complex chitinolytic system in exponentially growing mycelium of Mucor rouxii: properties and function.
An hypothesis is presented for the function of the complex chitinolytic system of the fungal hypha in branching and, possibly, also in apical growth. Expand
Characterization of chitinase and chitobiase produced by the entomopathogenic fungus
Extracellular fluids from Metarhizium anisopliae grown on chitin as the sole carbon source contained distinct chit inase and chitobiase activities, which possessed simple glycosidase activity against p -nitrophenyl-acetylglucosaminide and hydrolyzed di-, tri-, and tetrasaccharides to N -acetyl glucosamine. Expand


Inhibition and activation of mannan synthesis in Saccharomyces cerevisiae spheroplast lysates
The results indicate the existence of a protein inhibitor of mannan synthesis whose inhibitory activity in spheroplast lysates may be modulated by preincubation at low temperature or by varying the available Mn2+ concentration. Expand
The Eukaryotic Microbial Cell
Candida and candidosis
A comprehensive and critical review of the medical and scientific literature on Candida infections by a leading authority in the field. Covers all aspects of the subject, including epidemiology,Expand
Papulacandin B: an inhibitor of glucan synthesis in yeast spheroplasts.
It is concluded that papulacandin B and probably also echinocand in B inhibit glucan synthesis during cell-wall synthesis, and thus cause lysis of cells by osmotic rupture. Expand
A rapid and sensitive assay for chitinase using tritiated chitin.
Radioactive chitin, prepared by acetylation of chitosan with tritiated acetic anhydride, was used as substrate in a rapid and extremely sensitive assay for chitInase, a result that cannot be attributed to an artifact of the method, to inhibition by product, or to instability of the enzyme. Expand
The results indicate that polyoxin B acts as an competitive inhibitor of chitin synthetase, which is concerned in the synthesis of cell-wall chitIn of A. kikuchiana, and suggest that the polyoxin-resistance of this fungus is caused by a lowered penetration of the antibiotic through the cell membrane into the enzyme site. Expand
Cell wall chemistry, morphogenesis, and taxonomy of fungi.