Variation in relative substrate specificity of bifunctional β-D-xylosidase/α-L-arabinofuranosidase by single-site mutations: Roles of substrate distortion and recognition


To probe differential control of substrate specificities for 4-nitrophenyl-α-L-arabinofuranoside (4NPA) and 4-nitrophenyl-β-D-xylopyranoside (4NPX), residues of the glycone binding pocket of GH43 β-D-xylosidase/α-L-arabinofuranosidase from Selenomonas ruminantium were individually mutated to alanine. Although their individual substrate specificities (kcat… (More)


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