Variation in Virulence of Plasmodiophora brassicae in Japan Tested with Clubroot-resistant Cultivars of Chinese Cabbage (Brassica rapa L. ssp. pekinensis)

  title={Variation in Virulence of Plasmodiophora brassicae in Japan Tested with Clubroot-resistant Cultivars of Chinese Cabbage (Brassica rapa L. ssp. pekinensis)},
  author={Yasuhisa Kuginuki and Hiroaki Yoshikawa and Masashi Hirai},
  journal={European Journal of Plant Pathology},
The differential hosts of Williams (1966) and the European Clubroot Differential (ECD) (Buczacki et al., 1975) have been used commonly to identify populations of Plasmodiophora brassicae, which causes clubroot disease in Brassica crops. However, some of these hosts showed intermediate and fluctuating scores to most populations from Japan. Therefore, these hosts could not be used to provide a clear classification in Japan. We have tried to clarify the genetic diversity in pathogenicity of P… 
Pathogenic and genetic diversity in Plasmodiophora brassicae (clubroot) from Japan
The result implies an interesting genetic relationship among Williams’ races, among pathotypes determined using CR cultivars of Chinese cabbage and among populations from crops and C. flexuosa.
Virulence and pathotype classification of Plasmodiophora brassicae populations collected from clubroot resistant canola (Brassica napus) in Canada
A total of 17 pathotypes were detected using the CCD Set, compared with five pathotypes using the system of Williams and Somé et al., suggesting that the C CD Set has a greater differentiating capacity.
Pathotype Classification of Plasmodiophora brassicae Isolates Using Clubroot-Resistant Cultivars of Chinese Cabbage
Findings suggest that the four differential hosts of Chinese cabbage and four pathotype groups of P. brassicae might provide an efficient screening system for resistant cultivars and a new foundation of breeding strategies for CR Chinese cabbage.
Colonization by two isolates of Plasmodiophora brassicae with differing pathogenicity on a clubroot-resistant cultivar of Chinese cabbage (Brassica rapa L. subsp. pekinensis)
A commercial clubroot-resistant F1 cultivar of Chinese cabbage (Brassica rapa L. subsp. pekinensis), Kukai 70, is resistant to an isolate of populations of Plasmodiophora brassicae from Hagi (HG)
Resistance to Plasmodiophora brassicae in Brassica rapa and Brassica juncea genotypes From China.
Most inbred lines of Chinese cabbage and all inbreeding lines of pak choi and mustard were susceptible to all three pathotypes but their susceptibility was lower to pathotype 3 than to pathotypes 5 and 6, which were both highly pathogenic on most of the tested genotypes.
Resistance of Cultivars of Chinese Cabbage to Plasmodiophora brassicae Isolates of Several Races Collected in Korea
The results suggest that the breakdown of CR in Chinese cabbage has already occurred in cultivation areas of Korea and resistance source introduced in CR cultivars may be very limited.
Chinese cabbage (Brassicarapa ssp. pekinensis) – a valuable source of resistance to clubroot (Plasmodiophora brassicae)
Two hybrid lines combined high levels of resistance with appropriate plant morphology, good seed quality traits and a stable chromosome number and arrangement and offered opportunities of further improvement of the breeding process.
Emergence of new virulence phenotypes of Plasmodiophora brassicae on canola (Brassica napus) in Alberta, Canada
The identification of new virulence phenotypes of P. brassicae capable of overcoming genetic resistance underscores the need for increased stewardship of resistance sources.
Relation between pathogenicity and genetic variation within Plasmodiophora brassicae
The relation between diversity of pathogenicity on clubroot-resistant (CR) cultivars of Chinese cabbage and DNA polymorphisms in 17 populations of Plasmodiophora brassicae from cruciferous plants was examined by inoculation tests and random amplified polymorphic DNA (RAPD) analysis.
Inheritance of resistance to the newly identified Plasmodiophora brassicae pathotypes in Brassica napus L.
Two doubled haploid (DH) populations, developed by crossing a canola line carrying resistance to 3H, 3A, and 5X to two canola linescarrying resistance to3H, were studied for resistance to these three pathotypes, suggesting that pyramiding of multiple resistance genes in canola is possible.


Variation for virulence on Brassica napus L. amongst Plasmodiophora brassicae collections from France and derived single‐spore isolates
Variation for virulence was examined amongst 20 field collections of Plasmodiophora brassicae from France and the fractionation of different individual pathotypes from one original spore suspension confirmed the genetic heterogeneity of field populations of P. Brassicae.
A genetic map for Brassica oleracea based on RFLP markers detected with expressed DNA sequences and mapping of resistance genes to race 2 of Plasmodiophora brassicae (Woronin)
Leaf morphology and biennial flowering appeared to segregate as single Mendelian traits, but only leaf morphology could be linked to other markers, and two dominant QTLs for resistance to race 2 of the clubroot disease causal agent were identified.
Detection and analysis of QTLs based on RAPD markers for polygenic resistance to Plasmodiophora brassicae Woron in Brassica oleracea L.
QTL analysis suggests the existence of at least two genetic mechanisms implicated in the resistance phenomenon, which is consistent with previous reports on clubroot-resistant kale and cauliflower.
Association of RFLP markers with trait loci affecting clubroot resistance and morphological characters in Brassica oleracea L.
It was found that in some instances, RFLP markers from the same or closely linked chromosomal regions were associated with both clubroot resistance and morphological traits, which should facilitate the selection of desired disease resistant morphotypes.
Plasmodiophora brassicae: aspects of pathogenesis and resistance inBrassica oleracea
Clubroot is one of the most damaging diseases in Brassica oleracea crops world-wide and progress in the understanding of the inheritance of resistance is being made through the use of single-spore isolates of the pathogen, and theUse of molecular markers for resistance genes.
Characterization of isolates derived from single resting spores of Plasmodiophora brassicae and studies of their interaction.
A technique for inoculation of Brassica seedlings with single resting spores of Plasmodiophora brassicae is described, which found that when two isolates were mixed together, spores of one isolate restricted infection by spores of the other isolate.
Technique for single spore infection by Plasmodiophora brassicae
Breeding of Chinese cabbage with clubroot resistance in Japan
  • ISHS Symposium on Brassicas, Abstracts of 9th Crucifer Genetics Workshop (pp
  • 1994