Variable protection against experimental broiler necrotic enteritis after immunization with the C-terminal fragment of Clostridium perfringens alpha-toxin and a non-toxic NetB variant

@article{Costa2016VariablePA,
  title={Variable protection against experimental broiler necrotic enteritis after immunization with the C-terminal fragment of Clostridium perfringens alpha-toxin and a non-toxic NetB variant},
  author={S{\'e}rgio P. Fernandes da Costa and D. Mot and S. Geeraerts and M. Bokori-Brown and F. Van Immerseel and R. Titball},
  journal={Avian Pathology},
  year={2016},
  volume={45},
  pages={381 - 388}
}
ABSTRACT Necrotic enteritis toxin B (NetB) is a pore-forming toxin produced by Clostridium perfringens and has been shown to play a key role in avian necrotic enteritis, a disease causing significant costs to the poultry production industry worldwide. The aim of this work was to determine whether immunization with a non-toxic variant of NetB (NetB W262A) and the C-terminal fragment of C. perfringens alpha-toxin (CPA247–370) would provide protection against experimental necrotic enteritis… Expand
Oral vaccination of broiler chickens against necrotic enteritis using a non-virulent NetB positive strain of Clostridium perfringens type A.
TLDR
Testing a non-virulent NetB producing strain of C. perfringens (nvNetB+), with or without adjuvants, as an orally administered live vaccine fornecrotic enteritis in chickens and turkeys found there was milder intestinal pathology upon disease challenge. Expand
Immunization with subunits of a novel pilus produced by virulent Clostridium perfringens strains confers partial protection against necrotic enteritis in chickens.
TLDR
Western blot experiments using serum from immunized birds were performed, providing the first experimental evidence to suggest that this locus may in fact encode a functional pilus structure. Expand
Evaluation of a toxoid fusion protein vaccine produced in plants to protect poultry against necrotic enteritis
TLDR
Results indicate that a plant-produced PlcC-NetB toxoid is a promising vaccine candidate for controlling NE in poultry. Expand
Evaluation of a toxoid fusion protein vaccine produced in plants to protect poultry against necrotic enteritis
Background: Necrotic enteritis (NE) is caused by type A strains of the bacterium Clostridium perfringens. Total global economic losses to the poultry industry due to NE is estimated to be over twoExpand
Immunization with oral and parenteral subunit chimeric vaccine candidate confers protection against Necrotic Enteritis in chickens.
TLDR
The results showed that birds immunized both parenterally and orally with recombinant chimeric vaccine were significantly protected against the severity of lesion in the intestinal tract, but the protection provided with the injectable form of the antigen was greater than that of the oral form. Expand
Salmonella-vectored vaccine delivering three Clostridium perfringens antigens protects poultry against necrotic enteritis
TLDR
The presence of Fba on the cell surface suggests that Fba may function as an adhesin in necrotic enteritis, and it is demonstrated that both toxins and Fba are present on the C. perfringens cell surface. Expand
silico design and in vitro analysis of a recombinant trivalent fusion protein candidate vaccine targeting virulence factor of Clostridium perfringens.
TLDR
It is suggested that combination of metallopeptidase protein along with alpha toxin and NetB toxins is a potent immunogen which is able to neutralize the toxicity of crude extracellular toxins. Expand
Vaccination against pathogenic clostridia in animals: a review
TLDR
Different kinds of clostridial toxins and the vaccines that are experimentally or practically available are reviewed and a short description on each vaccine focusing on its applications, advantages, and disadvantages are given. Expand
Design and Expression Optimization of a Chimeric Derivative of NetB, Alpha-Toxin and Metallopeptidase Proteins as a Subunit Vaccine Against Clostridium perfringens
Avian necrotic enteritis (NE) is a multi-virulence disease caused by the bacterium Clostridium perfringens. Several toxins of this bacteria are components of different candidate vaccines, and haveExpand
Vaccine Production to Protect Animals Against Pathogenic Clostridia
TLDR
This review discusses the toxins, diseases, and toxoid production processes of the most common pathogenic Clostridium species, including Clstridium botulinum, Clostridgeium tetani, Clastridium perfringens, ClOSTridium chauvoei, Cl ostridium septicum, clostridia novyi and Clost Ridium hemolyticum. Expand
...
1
2
...

References

SHOWING 1-10 OF 48 REFERENCES
Vaccination with Clostridium perfringens recombinant proteins in combination with Montanide™ ISA 71 VG adjuvant increases protection against experimental necrotic enteritis in commercial broiler chickens.
TLDR
It is suggested that vaccination with clostridial recombinant proteins, particularly NetB toxin or PFO, in combination with ISA 71 VG enhances protective immunity against experimental necrotic enteritis in broiler chickens. Expand
Oral immunization of broiler chickens against necrotic enteritis with an attenuated Salmonella vaccine vector expressing Clostridium perfringens antigens.
TLDR
This study showed the oral immunizing ability of two C. perfringens antigens against NE in broiler chickens through an attenuated Salmonella vaccine vector. Expand
Immunization of Broiler Chickens against Clostridium perfringens-Induced Necrotic Enteritis
TLDR
There are certain secreted proteins, in addition to alpha-toxin, that are involved in immunity to NE in broiler chickens, which are concluded to be uniquely produced by virulent C. perfringens. Expand
A genetically engineered vaccine against the alpha-toxin of Clostridium perfringens protects mice against experimental gas gangrene.
TLDR
Immunization with Cpa247-370 provided protection in a mouse model against at least 10 LD100 doses of C. perfringens type A and confirmed the essential role of this toxin in the pathogenesis of gas gangrene. Expand
Immunization of Broiler Chickens Against Clostridium perfringens–Induced Necrotic Enteritis Using Purified Recombinant Immunogenic Proteins
TLDR
This study demonstrated the partial efficacy of additional secreted proteins in immunity of broiler chickens to NE and showed that there may be differences in the protective ability of immunogens depending on the infecting C. perfringens strain. Expand
Vaccination with recombinant NetB toxin partially protects broiler chickens from necrotic enteritis
TLDR
It is suggested that vaccination with NetB alone may not be sufficient to protect birds from necrotic enteritis in the field, but that in combination with other cellular or cell-free antigens it can significantly protect chickens from disease. Expand
Recombinant Attenuated Salmonella enterica Serovar Typhimurium Expressing the Carboxy-Terminal Domain of Alpha Toxin from Clostridium perfringens Induces Protective Responses against Necrotic Enteritis in Chickens
TLDR
Results indicate that oral RASV expressing an alpha toxin C-terminal peptide induces protective immunity against NE, and three-day-old broiler chicks were orally immunized with 109 CFU of the vaccine strain and developed alpha toxin-neutralizing serum antibodies. Expand
Immunization with recombinant alpha toxin partially protects broiler chicks against experimental challenge with Clostridium perfringens.
TLDR
Results suggest that alpha toxin may serve as an effective immunogen, and, as such, may play a role in pathogenesis of necrotic enteritis in poultry. Expand
A field study of naturally occurring specific antibodies against Clostridium perfringens alpha toxin in Norwegian broiler flocks.
TLDR
The results showed that the flocks with high titers of specific maternal antibodies against PLC had lower mortality during the production period than flocksWith low titers, and the progenies from the oldest hens had significantly higher levels of specific antibodies than the chicks from younger hens. Expand
A new method for the experimental production of necrotic enteritis and its use for studies on the relationships between necrotic enteritis, coccidiosis and anticoccidial vaccination of chickens
TLDR
Immunization with a live, pentavalent, attenuated anticoccidial vaccine (Paracox-5), by virtue of its protection against clinical coccidiosis due to the E. maxima challenge, indirectly protected birds against a subsequent challenge with virulent C. perfringens. Expand
...
1
2
3
4
5
...