Variable product specificity of solanesyl pyrophosphate synthetase.

  title={Variable product specificity of solanesyl pyrophosphate synthetase.},
  author={Hirofumi Fujii and Hiroshi Sagami and Takako Koyama and Kyozo Ogura and Shuichi Seto and Tsuneo Baba and Charles M. Allen},
  journal={Biochemical and biophysical research communications},
  volume={96 4},
Enzymatic hydrolysis of polyprenyl pyrophosphates.
Dynamic interaction between components of hexaprenyl diphosphate synthase from Micrococcus luteus BP-26.
Evidence was obtained to show that farnesyl diphosphate (FPP) binds to the components to form an aggregate, A-B-FPP-Mg2+, which probably represents an intermediary state of enzyme catalysis.
Isoprenyl diphosphate synthases.
Decaprenyl Diphosphate Synthesis in Mycobacterium tuberculosis
This is the first report of a bacterial Z-prenyl dphosphate synthase that preferentially utilizes an allylic diphosphate primer having the alpha-isoprene unit in the Z configuration, indicating that Rv1086 (omega,E,Z-farnesyl diph phosphate synthase) and Rv2361c act sequentially in the biosynthetic pathway that leads to the formation of decaprenyl phosphate in M. tuberculosis.
Conversion of Product Specificity of Archaebacterial Geranylgeranyl-diphosphate Synthase
Sequence analysis revealed that each mutant contained a few amino acid substitutions and that the mutation of Phe-77, which is located on the fifth amino acid upstream from the first aspartate-rich consensus motif, is the most effective for elongating the ultimate product.


Heptaprenyl pyrophosphate synthetase from Bacillus subtilis.
Solanesyl pyrophosphate synthetase from Micrococcus lysodeikticus.
An enzyme preparation from young shoots of potato was found to hydrolyze the polyprenyl pyrophosphates effectively to give the corresponding prenols.
Biosynthesis of bacterial menaquinones: the membrane-associated 1,4-dihydroxy-2-naphthoate octaprenyltransferase of Escherichia coli.
The prenylation of of 1,4-dihydroxy-2-naphthoate was studied in a strain of E. coli which accumulates octaprenyl pyrophosphate, the natural precursor of the menaquinone side chain, and the octAPrenyltransferase was shown to be membrane bound and to require magnesium ions for optimal activity.
Ubiquinone and vitamin K in bacteria. 2. Intracellular distribution in Escherichia coli and Micrococcus lysodeikticus.
Evidence is presented that vitamin K and ubiquinone are similarly located, which would suggest that they play a part in respiratory processes, as has been suggested for animal tissues.
Distribution of Menaquinones in Aerobic Micrococcaceae
It is shown that several menaquinone patterns, possibly of taxonomic significance, may be found in Micrococcaceae, notably Staphylococcus aureus.