Variable deletion and duplication at recombination junction ends: implication for staggered double-strand cleavage in class-switch recombination.

@article{Chen2001VariableDA,
  title={Variable deletion and duplication at recombination junction ends: implication for staggered double-strand cleavage in class-switch recombination.},
  author={Xuemei Chen and Kazuo Kinoshita and T. Honjo},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2001},
  volume={98 24},
  pages={13860-5}
}
Immunoglobulin class-switch recombination (CSR) gives rise to looped-out circular DNA of a cleaved S segment, which is lost eventually after cell divisions. To understand the molecular mechanism of S region cleavage during CSR, we constructed artificial CSR substrates in which inversion-type CSR takes place to retain the cleaved S segment. Sequencing analyses of recombinant clones of these substrates revealed that varying degrees of deletions and duplications exist at CSR breakpoints… CONTINUE READING