Variability in the immunodetection of His-tagged recombinant proteins.

  title={Variability in the immunodetection of His-tagged recombinant proteins.},
  author={Natasa Debeljak and Laurie Feldman and Kerry L. Davis and Radovan Komel and Arthur J. Sytkowski},
  journal={Analytical biochemistry},
  volume={359 2},
Labeling of recombinant proteins with polypeptide fusion partners, or affinity tagging, is a useful method to facilitate subsequent protein purification and detection. Poly-histidine tags (His-tags) are among the most commonly used affinity tags. We report strikingly variable immunodetection of two His-tagged recombinant human erythropoietins (Epo): wild type Epo (Epo(wt)) and Epo containing an R103A mutation (Epo(R103A)). Both were engineered to contain a C-terminal six residue His-tag. The… CONTINUE READING
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