Variability and molecular typing of the woody-tree infecting prunus necrotic ringspot ilarvirus

@article{Vakov2000VariabilityAM,
  title={Variability and molecular typing of the woody-tree infecting prunus necrotic ringspot ilarvirus},
  author={D. Va{\vs}kov{\'a} and K. Petrzik and R. Kare{\vs}ov{\'a}},
  journal={Archives of Virology},
  year={2000},
  volume={145},
  pages={699-709}
}
Summary. The 3′-part of the movement protein gene, the intergenic region and the complete coat protein gene of sixteen isolates of Prunus necrotic ringspot virus (PNRSV) from five different host species from the Czech Republic were sequenced in order to search for the bases of extensive variability of viroses caused by this pathogen. According to phylogenetic analyses all the 46 isolates sequenced to date split into three main groups, which correlated to a certain extend with their geographic… Expand
MOLECULAR VARIABILITY OF THE COAT PROTEIN GENE OF Prunus necrotic ringspot virus ISOLATES
TLDR
The results indicated no association between the host species or the geographic origin and the PNRSV CP sequence specificity, which allowed for clustering them into the previously reported PV32-I, PV96-II and PE5-III phylogroups. Expand
Phylogeny of isolates of Prunus necrotic ringspot virus from the Ilarvirus Ringtest and identification of group-specific features
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Although no correlation was found between the sequence and host or geographic origin, there was a general trend for severe isolates to cluster into one group and group-specific features have been identified for discrimination between virus strains. Expand
Genetic diversity of the movement and coat protein genes of South American isolates of Prunus necrotic ringspot virus
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The study of the distribution of synonymous and nonsynonymous changes along both open reading frames revealed that most amino acid sites are under the effect of negative purifying selection. Expand
Biological and molecular characterization of Prunus necrotic ringspot virus isolates from three rose cultivars
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The sequence of the MP gene of the P NRSV-R1 isolate was determined, the first such results for a rose-derived PNRSV isolate, and Melonowa Żółta appeared to be the most useful test plants for the differentiation of isolate-specific pathogenicity. Expand
Genetic Diversity of Prunus necrotic ringspot virus Isolates Within a Cherry Orchard in New York.
TLDR
Phylogenetic analyses showed a clustering of PNRSV isolates from cherry trees in New York in the predominant group PV-96, and sequence analysis of the CP gene indicated a population structure consisting of one predominant molecular variant for 10 isolates and six minor molecular variants for seven isolates. Expand
Detection and genetic diversity of Prunus necrotic ringspot virus in the Niagara Fruit Belt, Canada
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This study represents the first characterization of Prunus necrotic ringspot virus in Canada and suggests these 31 isolates belong to two groups, PV96 and PV32, with the former as the predominant group. Expand
Molecular Evidence for Recombination in Prunus Necrotic Ringspot Virus
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TLDR
The use of RECCO algorithm and Tajima Neutrality test implemented in MEGA4 program indicated the occurrence of a high level of deletion/insertion events in the sequences of Prunus necrotic ringspot virus. Expand
Genetic diversity of Prunus necrotic ringspot virus infecting stone fruit trees grown at seven regions in China and differentiation of three phylogroups by multiplex RT-PCR
TLDR
A multiplex RT-PCR assay based on CP gene sequences was developed to differentiate isolates in three phylogroups, finding sequence variations around a hexanucleotide insertion at sites 124–129 in the CP gene in six PV32-type isolates from Prunus avium resulted in an alteration of secondary structure. Expand
Biological and molecular characterisation of Prunus necrotic ringspot virus isolates and possible approaches to their phylogenetic typing
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Seven isolates of Prunus necrotic ringspot virus (PNRSV) originating from Slovakia were subjected to test biological cherry rootstocks to determine gene observed of rour on chip-budded isolate clusters, mainly for the mild capsid symptoms protein (CP). Expand
DETECTION AND PHYLOGENETIC ANALYSIS OF PRUNUS NECROTIC RINGSPOT VIRUS ISOLATES FROM STONE FRUITS IN IRAN
TLDR
Neighbor-Joining phylogenetic trees were generated on the basis of the nt or aa data of the CP showing that the newly-sequenced isolates were placed within the previously defined PNRSV group known as PV-96-II, which comprises isolates from diverse geographical regions such as Poland, Uruguay, China, Chile, Montenegro, and the USA. Expand
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Single nucleotide and amino acid differences with strong correlations to serology and symptom types (pathotypes) were revealed and Sequence differences between serotypes and pathotypes were also reflected in the overall phylogenetic relationships between the isolates. Expand
Molecular Variability Among Isolates of Prunus Necrotic Ringspot Virus from Different Prunus spp.
ABSTRACT Viral sequences amplified by polymerase chain reaction from 25 isolates of Prunus necrotic ringspot virus (PNRSV), varying in the symptomatology they cause in six different Prunus spp., wereExpand
Evolutionary relationships in the ilarviruses: nucleotide sequence of prunus necrotic ringspot virus RNA 3.
TLDR
Comparison and phylogenetic analysis of both the MP and CP proteins revealed a higher relationship of A1MV to PNRSV, apple mosaic virus (ApMV) and PDV than any other member of the ilarvirus group. Expand
Detection of Prunus necrotic ringspot virus serotypes in herbaceous and Prunus hosts with a complementary RNA probe
Complementary DNAs (cDNAs) prepared from genomic RNA of a peach isolate of Prunus necrotic ringspot virus (PNRSV) were used to produce a 32 P-labeled complementary RNA (cRNA) probe that was capableExpand
Biophysical differences among prunus necrotic ringspot ilarviruses.
Intact nucleoproteins of several isolates of Prunus necrotic ringspot virus (PNRSV) from cherry, peach, almond, and hop resolved as three components in rate-zonal sucrose density gradients and as twoExpand
Prunus necrotic ringspot ilarvirus: nucleotide sequence of RNA3 and the relationship to other ilarviruses based on coat protein comparison.
TLDR
The RNA3 of prunus necrotic ringspot ilarvirus (PNRSV) has been cloned and its entire sequence determined and showed a high degree of similarity with those of tobacco streak virus (TSV), prune dwarf virus (PDV), apple mosaic virus (ApMV) and also alfalfa mosaicirus (AIMV). Expand
The complete nucleotide sequence of RNA 3 of a peach isolate of Prunus necrotic ringspot virus.
TLDR
The results in one instance confirm the close similarities observed at the biophysical and serological levels between these two viruses, but on the other hand call into question the nomenclature used to describe these viruses. Expand
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TLDR
The results support the idea that both features (metal-binding domain and highly conserved hairpin structures) are characteristics of ilarviruses and are probably involved in the peculiar 'genome activation' phenomenon described in these viruses. Expand
Characterization of sequences controlling the synthesis of alfalfa mosaic virus subgenomic RNA in vivo.
TLDR
Engineering of point mutations and small deletions in RNA 3 around the transcription start site for RNA 4 synthesis revealed elements important for sg promoter activity with similarity to sequences conserved in sg promoters of alpha-like viruses. Expand
Early and late functions of alfalfa mosaic virus coat protein can be mutated separately.
TLDR
Early and late functions of CP can be mutated separately, indicating that different domains of CP are involved in the three functions investigated, including genome activation, plus-strand RNA accumulation, and cell-to-cell spread of the virus. Expand
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