Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells.

@article{Wulhfard2010ValproicAE,
  title={Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells.},
  author={Sarah Wulhfard and L. Baldi and David L. Hacker and F. M. Wurm},
  journal={Journal of biotechnology},
  year={2010},
  volume={148 2-3},
  pages={128-32}
}
Valproic acid (VPA) is a small molecule that inhibits histone deacetylase activity. Here we report that VPA increases recombinant mRNA and protein levels in transiently transfected CHO DG44 cells. In the presence of VPA, transient recombinant antibody yields of up to 40 mg/L were achieved in simple batch cultures. The steady-state levels of the IgG light and heavy chain mRNAs were nearly 10 times higher than in the untreated control transfection even though the level of transfected plasmid DNA… CONTINUE READING

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