Validity of endothelial cell analysis methods and recommendations for calibration in Topcon SP-2000P specular microscopy.


PURPOSE To report on the calibration of the Topcon SP-2000P specular microscope and the Endothelial Cell Analysis Module of the IMAGEnet 2000 software, and to establish the validity of the different endothelial cell density (ECD) assessment methods available in these instruments. METHODS Using an external microgrid, we calibrated the magnification of the… (More)


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