Validation of the specificity and sensitivity of species-specific primers that provide a reliable molecular diagnostic for Xiphinema diversicaudatum, X. index and X. vuittenezi

@article{Hbschen2004ValidationOT,
  title={Validation of the specificity and sensitivity of species-specific primers that provide a reliable molecular diagnostic for Xiphinema diversicaudatum, X. index and X. vuittenezi},
  author={Judith M. H{\"u}bschen and Lilo Kling and Ulrike Ipach and Volker Zinkernagel and Nathalie Bosselut and Daniel Esmenjaud and Derek J. F. Brown and Roy Neilson},
  journal={European Journal of Plant Pathology},
  year={2004},
  volume={110},
  pages={779-788}
}
Xiphinema diversicaudatum and X. index are vector nematode species of economic importance in viticulture regions as they can transmit Arabis Mosaic, Grapevine Fanleaf and Strawberry Latent Ringspot viruses to grapevine. Wang et al. (2003) designed species-specific diagnostic primers from ribosomal genes for both these vector species as well as a vector and a non-vector species X. italiae and X. vuittenezi, respectively. Our study aimed to confirm the specificity and determine the sensitivity… CONTINUE READING
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