Validation of the single-platform ISHAGE method for CD34(+) hematopoietic stem and progenitor cell enumeration in an international multicenter study.

@article{Gratama2003ValidationOT,
  title={Validation of the single-platform ISHAGE method for CD34(+) hematopoietic stem and progenitor cell enumeration in an international multicenter study.},
  author={Jan Willem C. Gratama and J. K. Kraan and Michael Keeney and David R. Sutherland and V. Granger and David Barnett},
  journal={Cytotherapy},
  year={2003},
  volume={5 1},
  pages={55-65}
}
BACKGROUND Flow cytometric enumeration of CD34+ hematopoietic sterm and progenitor cells (HPC) is the reference point for undertaking apheresis and evaluation of adequacy for PBSC engraftment. An external quality assurance (EQA) scheme for CD34+ HPC enumeration has been operational in Belgium, Netherlands and Luxemburg (Benelux) since 1995. Within this group, a multicenter survey was held to validate the state-of-the-art methodology, i.e., multiparametric definition of HPC based on light… CONTINUE READING

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