Validation of testing and interpretation protocols for low template DNA samples using AmpFlSTR Identifiler.

@article{Caragine2009ValidationOT,
  title={Validation of testing and interpretation protocols for low template DNA samples using AmpFlSTR Identifiler.},
  author={Theresa A Caragine and Rebecca S. Mikulasovich and Jeannie Tamariz and Ewelina J Bajda and James Sebestyen and Howard J Baum and M Gonzalez Prinz},
  journal={Croatian medical journal},
  year={2009},
  volume={50 3},
  pages={250-67}
}
AIM To test the reliability, robustness, and reproducibility of short tandem repeat (STR) profiling of low template DNA (LT-DNA) when employing a defined set of testing and interpretation parameters. METHODS DNA from known donors was measured with a quantitative real time polymerase chain reaction (PCR) assay that consistently detects less than 1 pg/microL of DNA within a factor of 0.3. Extracts were amplified in triplicate with AmpFlSTR Identifiler reagents under enhanced PCR conditions… CONTINUE READING
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