Validation of reference genes for reverse transcription real‐time quantitative PCR analysis in the deep‐sea bacterium Shewanella psychrophila WP2

@article{Liu2018ValidationOR,
  title={Validation of reference genes for reverse transcription real‐time quantitative PCR analysis in the deep‐sea bacterium Shewanella psychrophila WP2},
  author={Shunzhang Liu and Canxing Meng and Guanpeng Xu and Huahua Jian and F. Wang},
  journal={FEMS Microbiology Letters},
  year={2018},
  volume={365},
  pages={1–5}
}
  • Shunzhang Liu, Canxing Meng, +2 authors F. Wang
  • Published 2018
  • Biology, Medicine
  • FEMS Microbiology Letters
  • &NA; Reference genes are critical to obtain reliable results of reverse transcription real‐time quantitative PCR (RT‐qPCR), which is widely used for relative quantification of gene expression. In this study, we evaluated the validity of seven candidate reference genes for normalization in RT‐qPCR analysis in the deep‐sea bacterium Shewanella psychrophila WP2 under different environmental conditions. Among the set of genes investigated, gyrA, 16S rRNA and rho were identified as the most suitable… CONTINUE READING
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