Validation of real-time PCR for laboratory diagnosis of Acanthamoeba keratitis.

  title={Validation of real-time PCR for laboratory diagnosis of Acanthamoeba keratitis.},
  author={P. Thompson and Regis Paul Kowalski and Robert M Q Shanks and Yitzchak J. Gordon},
  journal={Journal of clinical microbiology},
  volume={46 10},
Confirmation of Acanthamoeba keratitis by laboratory diagnosis is the first step in the treatment of this vision-threatening disease. Two real-time PCR TaqMan protocols (the Rivière and Qvarnstrom assays) were developed for the detection of genus-specific Acanthamoeba DNA but lacked clinical validation. We have adapted these assays for the Cepheid SmartCycler II system (i) by determining their real-time PCR limits of detection and amplification efficiencies, (ii) by determining their ability to… CONTINUE READING

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