Validation of real-time PCR for laboratory diagnosis of Acanthamoeba keratitis.

@article{Thompson2008ValidationOR,
  title={Validation of real-time PCR for laboratory diagnosis of Acanthamoeba keratitis.},
  author={P. Thompson and Regis Paul Kowalski and Robert M Q Shanks and Yitzchak J. Gordon},
  journal={Journal of clinical microbiology},
  year={2008},
  volume={46 10},
  pages={3232-6}
}
Confirmation of Acanthamoeba keratitis by laboratory diagnosis is the first step in the treatment of this vision-threatening disease. Two real-time PCR TaqMan protocols (the Rivière and Qvarnstrom assays) were developed for the detection of genus-specific Acanthamoeba DNA but lacked clinical validation. We have adapted these assays for the Cepheid SmartCycler II system (i) by determining their real-time PCR limits of detection and amplification efficiencies, (ii) by determining their ability to… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.
16 Citations
20 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 16 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 20 references

Similar Papers

Loading similar papers…