Validation of real-time PCR analyses for line-specific quantitation of genetically modified maize and soybean using new reference molecules.

@article{Shindo2002ValidationOR,
  title={Validation of real-time PCR analyses for line-specific quantitation of genetically modified maize and soybean using new reference molecules.},
  author={Yoichiro Shindo and Hideo Kuribara and Takeshi Matsuoka and Satoshi Futo and Chihiro Sawada and Jinji Shono and Hiroshi Akiyama and Yukihiro Goda and Masatake Toyoda and Akihiro Hino},
  journal={Journal of AOAC International},
  year={2002},
  volume={85 5},
  pages={1119-26}
}
Novel analytical methods based on real-time quantitative polymerase chain reactions by use of new reference molecules were validated in interlaboratory studies for the quantitation of genetically modified (GM) maize and soy. More than 13 laboratories from Japan, Korea, and the United States participated in the studies. The interlaboratory studies included 2 separate stages: (1) measurement tests of coefficient values, the ratio of recombinant DNA (r-DNA) sequence, and endogenous DNA sequence in… CONTINUE READING