Validation of increased blood storage times with the T-SPOT.TB assay with T-Cell Xtend reagent in individuals with different tuberculosis risk factors.

@article{Wang2012ValidationOI,
  title={Validation of increased blood storage times with the T-SPOT.TB assay with T-Cell Xtend reagent in individuals with different tuberculosis risk factors.},
  author={Shu-hua Wang and Samuel S Stew and Joshua C. Cyktor and Bridget Carruthers and Joanne Turner and Blanca I Restrepo},
  journal={Journal of clinical microbiology},
  year={2012},
  volume={50 7},
  pages={2469-71}
}
We compared the performance of the T-SPOT.TB assay with blood used within 0 to 3.5 h after collection (control) to its performance with blood stored for 0 to 3.5, 5 to 8, 18 to 21, or 31 to 33 h with the addition of T-Cell Xtend (experimental), using samples from 154 participants. The 95.4% concordance between paired specimens indicated that blood can be stored for up to 33 h prior to T-SPOT.TB testing. 

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