Validation of high throughput screening of human sera for detection of anti-PA IgG by Enzyme-Linked Immunosorbent Assay (ELISA) as an emergency response to an anthrax incident.

@article{Semenova2017ValidationOH,
  title={Validation of high throughput screening of human sera for detection of anti-PA IgG by Enzyme-Linked Immunosorbent Assay (ELISA) as an emergency response to an anthrax incident.},
  author={Vera A. Semenova and Evelene Steward-Clark and Panagiotis Maniatis and Monica F Epperson and Amit Sabnis and Jarad M. Schiffer},
  journal={Biologicals : journal of the International Association of Biological Standardization},
  year={2017},
  volume={45},
  pages={
          61-68
        }
}
To improve surge testing capability for a response to a release of Bacillus anthracis, the CDC anti-Protective Antigen (PA) IgG Enzyme-Linked Immunosorbent Assay (ELISA) was re-designed into a high throughput screening format. The following assay performance parameters were evaluated: goodness of fit (measured as the mean reference standard r2), accuracy (measured as percent error), precision (measured as coefficient of variance (CV)), lower limit of detection (LLOD), lower limit of… CONTINUE READING
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