Validation of endogenous internal real-time PCR controls in renal tissues.

  title={Validation of endogenous internal real-time PCR controls in renal tissues.},
  author={Xiangqin Cui and Juling Zhou and Jing Qiu and Martin R. Johnson and Michal M Mrug},
  journal={American journal of nephrology},
  volume={30 5},
BACKGROUND Endogenous internal controls ('reference' or 'housekeeping' genes) are widely used in real-time PCR (RT-PCR) analyses. Their use relies on the premise of consistently stable expression across studied experimental conditions. Unfortunately, none of these controls fulfills this premise across a wide range of experimental conditions; consequently, none of them can be recommended for universal use. METHODS To determine which endogenous RT-PCR controls are suitable for analyses of renal… CONTINUE READING
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