Validation of endogenous controls for quantitative gene expression analysis: application on brain cortices of human chronic alcoholics.

@article{Johansson2007ValidationOE,
  title={Validation of endogenous controls for quantitative gene expression analysis: application on brain cortices of human chronic alcoholics.},
  author={Sofia Johansson and Andrea Fuchs and Anna Okvist and Mohsen Karimi and Clive Harper and Therese Garrick and Donna Sheedy and Yasmin L Hurd and Georgy Bakalkin and Tomas J. Ekstr{\"o}m},
  journal={Brain research},
  year={2007},
  volume={1132 1},
  pages={20-8}
}
Real-time PCR is frequently used for gene expression quantification due to its methodological sensitivity and reproducibility. The gene expression is quantified by normalization to one or more reference genes, usually beta-actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPD) or to ribosomal RNA (18S). However, different environmental or pathological conditions might also influence the expression of normalizing genes, which could severely skew the interpretation of quantitative results… CONTINUE READING