Validation of an in vitro model for assessment of androstenedione hepatotoxicity using the rat liver cell line clone-9.

@article{Sahu2008ValidationOA,
  title={Validation of an in vitro model for assessment of androstenedione hepatotoxicity using the rat liver cell line clone-9.},
  author={Saura C. Sahu and Paddy L. Wiesenfeld and Chung S Kim and Ivan A. Ross and Philip P Sapienza and Richard N. L. Andrews Dr. Douglas J. Crawford - Brown Dr. Newell and Michael W O'donnell and Thomas J. Flynn},
  journal={Journal of applied toxicology : JAT},
  year={2008},
  volume={28 5},
  pages={703-9}
}
Androstenedione, a naturally occurring steroid hormone, has been used to enhance athletic performance. Little is known, however, about its hepatotoxicity. Clone-9 cells, a non-transformed epithelial cell line that was originally isolated from normal liver of a 4-week old Sprague-Dawley rat, were used as an in vitro model to assess the hepatotoxic potential of androstenedione. The cultures were treated with androstenedione for 24 h at 37 degrees C in 5% CO(2) at concentrations of 0-100 microg ml… CONTINUE READING