Validation of an in vitro method to determine infectivity of cryopreserved sporozoites in stabilates of Theileria spp.

@article{Wilkie2002ValidationOA,
  title={Validation of an in vitro method to determine infectivity of cryopreserved sporozoites in stabilates of Theileria spp.},
  author={Gwen M Wilkie and Erol Kirvar and C. G. Duncan Brown},
  journal={Veterinary parasitology},
  year={2002},
  volume={104 3},
  pages={199-209}
}
The infectivity of 15 cryopreserved Theileria spp. sporozoite stabilates was assessed semi-quantitatively by titration using naive peripheral blood mononuclear cells (PBM) in vitro in multi-well plates. Using the method described, the effective dilution, which would result in 50% of replicate wells infected (ED50), was calculated. The ED50 for 11 Theileria… CONTINUE READING