Validation of an in vitro biofilm model of supragingival plaque.

@article{Guggenheim2001ValidationOA,
  title={Validation of an in vitro biofilm model of supragingival plaque.},
  author={Bernhard Guggenheim and Elin Giertsen and Peter Michael Sch{\"u}pbach and Stuart Shapiro},
  journal={Journal of dental research},
  year={2001},
  volume={80 1},
  pages={363-70}
}
The study of biofilm structure and function mandates the use of model systems for which a host of environmental variables can be rigorously controlled. We describe a model of supragingival plaque containing Actinomyces naeslundii, Veillonella dispar, Fusobacterium nucleatum, Streptococcus sobrinus, and Streptococcus oralis wherein cells are cultivated anaerobically in a saliva-based medium on hydroxyapatite discs coated with a salivary pellicle, with material and pieces of apparatus common to… CONTINUE READING

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