Validation of an enzyme-linked immunosorbent assay for measuring vitellogenin in California halibut (Paralichthys californicus).

@article{VzquezBoucard2008ValidationOA,
  title={Validation of an enzyme-linked immunosorbent assay for measuring vitellogenin in California halibut (Paralichthys californicus).},
  author={Celia V{\'a}zquez-Boucard and Mario Alberto Burgos-Aceves and Fabiola Guadalupe Arcos-Ortega and Gerardo Alfonso Anguiano-Vega},
  journal={Environmental toxicology and chemistry},
  year={2008},
  volume={27 7},
  pages={1614-20}
}
Vitellogenin (VTG) is the major protein present in the plasma of females undergoing oogenesis. In males, the VTG gene normally is suppressed; however, synthesis of VTG can be induced by exposure to xenoestrogenic compounds. In the present study, an enzyme-linked immunosorbent assay was developed and validated to evaluate VTG levels in the California halibut (Paralichthys californicus). Vitellogenin and lipovitellin (LV) were identified in the plasma of 17 beta-estradiol-induced females and in… CONTINUE READING