Validation of an HPLC-UV method for analysis of Kaempferol-loaded nanoemulsion and its application to in vitro and in vivo tests.

A simple and reliable HPLC-UV method for Kaempferol (KPF) determination in a Kaempferol-loaded nanoemulsion (KPF-NE), samples from mucosa permeation/retention studies, and murine brain was developed and validated according to international guidelines. The analyses were performed on a reversed-phase C18 column at 35°C and under UV detection at 368nm. The… CONTINUE READING