Validation of an Automated Spectrophotometric Assay for the Determination of Cholinesterase Activity in Canine Serum

@article{Furlanello2006ValidationOA,
  title={Validation of an Automated Spectrophotometric Assay for the Determination of Cholinesterase Activity in Canine Serum},
  author={Tommaso Furlanello and Giulia Simonato and Marco Cald{\'i}n and Davide De Lorenzi and George Lubas and Daniele Bernardini and Laia Solano-Gallego},
  journal={Veterinary Research Communications},
  year={2006},
  volume={30},
  pages={723-733}
}
The determination of enzymatic activity of cholinesterase is a useful diagnostic method to detect exposure to anticholinesterase compounds in human and in veterinary medicine. We validated a modification of the Ellman method in canine serum and applied it to the diagnosis of dogs poisoned with anticholinesterase substances. The method used butyrylthiocholine as substrate and potassium hexacyanoferrate as chromophore. The reference range calculated on 60 clinically healthy dogs was set between… CONTINUE READING