Validation of a reference control for an SYBR-Green fluorescence assay-based real-time PCR for detection of bovine herpesvirus 5 in experimentally exposed bovine embryos.

@article{Cardoso2013ValidationOA,
  title={Validation of a reference control for an SYBR-Green fluorescence assay-based real-time PCR for detection of bovine herpesvirus 5 in experimentally exposed bovine embryos.},
  author={Tereza Cristina Cardoso and Camila Silva-Frade and Cilene Vidovix T{\'a}paro and Lucas Hidenori Okamura and Eduardo Furtado Flores},
  journal={Molecular and cellular probes},
  year={2013},
  volume={27 5-6},
  pages={237-42}
}
The objective of this study was to optimize an internal control to improve SYBR-Green-based qPCR to amplify/detect the BoHV-5 US9 gene in bovine embryos produced in vitro and experimentally exposed to the virus. We designed an SYBR-Green-based binding assay that is quick to perform, reliable, easily optimized and compares well with the published assay… CONTINUE READING