Validation of a real-time RT-PCR assay for sensitive and specific detection of classical swine fever.

@article{Hoffmann2005ValidationOA,
  title={Validation of a real-time RT-PCR assay for sensitive and specific detection of classical swine fever.},
  author={Bernd Hoffmann and Martin Beer and Christian Schelp and Horst Schirrmeier and Klaus Depner},
  journal={Journal of virological methods},
  year={2005},
  volume={130 1-2},
  pages={36-44}
}
A fully validated, ready-to-use, real-time reverse transcription-polymerase chain reaction (RT-PCR) assay, multiplexed for simultaneous detection of an internal control, for the simple and rapid diagnosis of classical swine fever (CSF) was developed. Primers and FAM-labeled TaqMan-probes specific for classical swine fever virus (CSFV) were selected from the consensus sequence of the 5' non-translated region (5' NTR) of 78 different CSFV strains. For determining analytical sensitivity, an in… CONTINUE READING

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