Validation of a quantitative SPR assay for recombinant FVIII.

  title={Validation of a quantitative SPR assay for recombinant FVIII.},
  author={A N McCormick and Meganne E. Leach and Geoffrey F. Savidge and Anwar Alhaq},
  journal={Clinical and laboratory haematology},
  volume={26 1},
Surface plasmon resonance was employed to establish a quantitative assay for recombinant FVIII (rFVIII) products using rFVIII as standard. The anti-FVIII monoclonal antibody ESH4 was immobilized onto a carboxymethyldextran surface. A range of rFVIII concentrations were injected over the surface and the binding response enhanced by the addition of a further monoclonal antibody ESH8. Validation using National Institute of Biological Standards and Controls (NIBSC) sixth International rFVIII… CONTINUE READING