Validation of a non-invasive blood-sampling technique for doubly-labelled water experiments.

@article{Voigt2003ValidationOA,
  title={Validation of a non-invasive blood-sampling technique for doubly-labelled water experiments.},
  author={Christian C Voigt and Otto von Helversen and Robert H. Michener and Thomas H. Kunz},
  journal={Journal of experimental zoology. Part A, Comparative experimental biology},
  year={2003},
  volume={296 2},
  pages={87-97}
}
Two techniques for bleeding small mammals have been used in doubly-labeled water (DLW) studies, including vena puncture and the use of starved nymphal stages of hematophagous reduviid bugs (Reduviidae, Hemiptera). In this study, we tested the validity of using reduviid bugs in doubly-labeled water experiments. We found that the isotope enrichment in initial blood samples collected with bugs was significantly lower compared to isotope enrichment in blood samples obtained using vena puncture. We… CONTINUE READING
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