Validation of a modified algorithm for the identification of yeast isolates using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS)

@article{Herendael2011ValidationOA,
  title={Validation of a modified algorithm for the identification of yeast isolates using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS)},
  author={Bruno van Herendael and Peggy Bruynseels and M. Bensaid and Tenuis Boekhout and Thierry de Ba{\`e}re and Ignace Surmont and A. H. Mertens},
  journal={European Journal of Clinical Microbiology & Infectious Diseases},
  year={2011},
  volume={31},
  pages={841-848}
}
Optimising antifungal treatment requires the fast and species-specific identification of yeast isolates. We evaluated a modified protocol for the rapid identification of clinical yeast isolates using matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) technology. First, we evaluated a simplified extraction procedure using 54 clinical yeast isolates. Second, we validated a new protocol with this simplified extraction procedure and lower identification threshold by analysing… CONTINUE READING
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