Validation of a modified PCR-based method for identifying mutant restricted ovulator chickens: substantiation of genotypic classification by phenotypic traits.

@article{Elkin2003ValidationOA,
  title={Validation of a modified PCR-based method for identifying mutant restricted ovulator chickens: substantiation of genotypic classification by phenotypic traits.},
  author={Robert G. Elkin and Yuan Zhong and Robert E. Porter and Rosemary L. Walzem},
  journal={Poultry science},
  year={2003},
  volume={82 4},
  pages={
          517-25
        }
}
Upon photostimulation, restricted ovulator (RO) female chickens exhibit endogenous hyperlipidemia, develop atherosclerotic lesions, and generally fail to lay eggs. This phenotype results from a point mutation in the gene specifying the very low density lipoprotein receptor (VLDLR), whose protein product normally mediates the massive oocytic uptake of egg yolk precursors from the circulation. Taking advantage of the single base change in the mutant VLDLR allele, a PCR-based method for the rapid… CONTINUE READING

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