• DOI:10.19080/JFSCI.2017.02.555600
• Corpus ID: 37515185

Validation of a Novel and Sensitive RP-HPLC Method for Simultaneous Determination of Cefixime Trihydrate and Sodium Benzoate in Powder for Oral Suspension Dosage Form

@inproceedings{Hassouna2017ValidationOA,
  title={Validation of a Novel and Sensitive RP-HPLC Method for Simultaneous Determination of Cefixime Trihydrate and Sodium Benzoate in Powder for Oral Suspension Dosage Form},
  author={Mem Hassouna},
  year={2017}
}

• M. Hassouna
• Published 31 March 2017
• Chemistry

CFX; is chemically known as (6R,7R)-7-[[(Z)-2-(2Aminothiazol-4-yl)-2-[(carboxymethoxy) imino] acetyl] amino]-3ethenyl-8-oxo-5-thia-1-azabicyclo [4.2.0] oct-2-ene-2-carboxylic acid trihydrate. It has a molecular formula of C16H15N5O7S2, 3H2O and a molecular weight of 507.5 [1,2] (Figure 1A). CFX is a white or almost white, slightly hygroscopic powder. Slightly soluble in water, soluble in methanol, sparingly soluble in anhydrous ethanol, practically insoluble in ethyl acetate. A 5% suspension in water has a pH of 2.6 to 4.1. Stored in airtight container to be protected from light [1,2]. CFX is bactericidal and is stable to hydrolysis by many beta-lactamases. Like other Cephalosporins, CFX possesses a mechanism of action similar to penicillins i.e. inhibition of transpeptidation process resulting in the formation of imperfect cell wall; osmotic drive from the outside isotonic environment of the host cell to the inside of the hypertonic bacterial cytoplasm and finally activation of the autolysin enzyme leading to the lysis of bacteria. Only 40 to 50% of an oral dose of CFX is absorbed from the gastrointestinal tract, whether taken before or after meals, although the rate of absorption may Abstract